Naby Keita had to be substituted early in the game against Brighton as the midfielder picked up another injury.
The Reds have now confirmed that it's a hamstring issue and that Keita will be unavailable for the Atletico clash.
Keita will undergo a scan on the issue to determine its severity but Klopp said: "If somebody feels the hamstring, it would be the first time that he is ready for the next game. So, I can’t see that."
Liverpool's midfield has looked light for a lot of the recent weeks but Thiago and Fabinho's imminent returns should help that.
Source: liverpoolfc.com
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